Secondary screening
Selected streptomycetes isolates were grown in a 250 ml Erlenmeyer
flask containing 100 ml of SCB at 28 ± 2°C for 3 days. Broth containing
streptomycetes were later extracted using ethyl acetate. Equal volume
of ethyl acetate was added to the 250 ml Erlenmeyer flask containing
100 ml of three days cultures. The flasks were later incubated at 28 ±
2°C with continuous agitation using an orbital shaker at 250 rpm for
one hour. After one hour, the organic phase was pipetted into the 50 ml
centrifuge tubes and was centrifuged at 4°C for 10 min at 8000 rpm. The
supernatant obtained were then filtered using 0.45 μm nitrocellulose
filter. Fifteen microliter of the filtrate which contained the crude
antifungal extract was inoculated onto each sterile filter paper discs