Others have employed PCR-DGGE or PCR-TGGE of rRNA gene segments to
differentiate individual wine yeast isolates. Given the discriminatory
power of T/DGGE this method works well as long as standards are run for each
potential species that one might observe in that environmental niche. This approach
is also useful to monitor primary enrichment cultures from wine or grape substrates.
Bae and co-workers used this approach to reveal the LAB on grape surfaces that were enriched via different media. In general, however, T/DGGE approaches for identification purposes are technically problematic since control strains must be present in the gel and band co-migration with known standards is not clear confirmation of identify.