However, frequent transfers (twice per month) with 10% (v/v) of inoculum in freshly prepared culture medium were found optimal to ensure re-growth. Alternatively, the isolate was stored in liquid nitrogen in the same medium containing 5% (w/v) DMSO.
However,frequent transfers (twice per month) with 10% (v/v) ofinoculum in freshly prepared culture medium were foundoptimal to ensure re-growth. Alternatively, the isolate wasstored in liquid nitrogen in the same medium containing5% (w/v) DMSO.