the quantitative determination of the investigated VACs was performed with a gas chromatograph,equipped with a flame ionization detector (FID) and with vertibond tm 5 ms capillary column thermo finingan,ltaly.the optimum conditions used throghout were shown in table 1
Essential oils were placed in test tube 10 mL and 2 mL of hexane was added. the sample solution in test tubes were mixed with vortex mixer and centrifuged. Then, lighter hexane layers were separated by dropper and keep in 2 mL vial. Finally, extracted was injected into GC-FID by 10 GC syringe