Phire Plant Direct PCR Kit (Finnzymes Oy, Finland) was used
to generate PCR products from herbarium specimens according
to the manufacturer’s instructions. To extract DNA,
a small piece of specimen was incubated in 20 ml dilution
buffer at room temperature for 3 min, and then 1.5 ml of the
supernatant were used as a template for a 50 ml PCR reaction.
LSU-rDNA region was amplified with primer pair LR0R and LR7