Extraction of sugars and inulin
Freeze-dried samples (1–5 g), containing about 1 g of inulintype
fructans, were weighed into a 100 mL screw cap bottle and
extracted with about 40 mL of hot water (about 60 8C). The pH of
solution was adjusted to 6.5–8.0 with 0.05 M KOH or 0.05 M HCl.
Final volume was made up to 50 mL with deionised water. The
mixture was incubated at 85 2 8C in shaking water-bath for
15 min