Many experimental and practical
approaches to stimulate the immune response of fish
were reported (Badran 1984, Abdel-Kader 1994 and Aly
et. al., 2000). Such immune response could be detected
either by the presence of specific antibodies in the blood
or by protection against infection. In the past, the
presence of antibodies in the blood is well revealed
when the immune system is stimulated by the injection
of the antigen, but not when given orally or by
immersion. After which, the trials were attempted to
increase the production of antibodies and prolonging
their presence in the blood by emulsifying the antigen in
adjuvant Krantz, et. al ( 1963) who used mineral oil
emulsion, Collins, et. al ( 1976) Fruend's incomplete
adjuvant (FIA) and Badran (1990) FIA and Fruend's
complete adjuvant (FCA). Moreover, several techniques
have been successfully used for fish vaccination. Such
techniques included injection immersion and oral routes