Given the known counting chamber volume in the blood count plate and the total cell suspension volume, the cotyledon cell number in total cell suspension was calculated as follows,W:W1 = X:A, then X = A * (W/W1), where W is the dry soybean seed weight, X is cotyledon cell number, W1 is the dry weight of a small amount of seed powder, and A is the cotyledon cell number in total cell suspension from this known weight. Cotyledon cell volume was obtained by dividing seed volume by number of cotyledon cells. Cell weight was obtained by dividing seed size by cotyledon cell number.