The amplification program consisted of 2 min at 95 C and 40
cycles of denaturation at 95 C for 30 s (for M13R2 and KS) or for
1 min (for CC1 and R5), annealing for 1 min (Table 1) and elongation
at 72 C (1.5 min for M13R2 and KS and 2 min for CC1 and R5),
followed by a final elongation at 72 C for 5 min (primer M13R2) or
7 min (primers KS, CC1 and R5). GeneAmp PCR System 2700
(Applied Biosystems, Foster City, CA, USA) was used.