3.1.2. Enzymatic hydrolysis
The degree of hydrolysis of native starch of PPW depends on
factors such as substrate concentration, type and concentration
of the enzyme used, and the process conditions such as pH,
temperature, etc. Firstly, the ability of each enzyme to degrade PPW carbohydrates to fermentable reducing sugars was tested.
The use of Ternamyl released 4.65 g L1, the Liquozyme
4.03 g L1 and Viscozyme only 0.7 g L1. Ternamyl and Liquozyme
are enzymes for starch liquefaction, and Viscozyme is used in saccharification.
These results indicate that the saccharification stage
alone was inadequate and a preliminary liquefaction stage was required.
For this reason, the use of enzyme combination was necessary
for an effective hydrolysis of PPW.
3.1.2. Enzymatic hydrolysisThe degree of hydrolysis of native starch of PPW depends onfactors such as substrate concentration, type and concentrationof the enzyme used, and the process conditions such as pH,temperature, etc. Firstly, the ability of each enzyme to degrade PPW carbohydrates to fermentable reducing sugars was tested.The use of Ternamyl released 4.65 g L1, the Liquozyme4.03 g L1 and Viscozyme only 0.7 g L1. Ternamyl and Liquozymeare enzymes for starch liquefaction, and Viscozyme is used in saccharification.These results indicate that the saccharification stagealone was inadequate and a preliminary liquefaction stage was required.For this reason, the use of enzyme combination was necessaryfor an effective hydrolysis of PPW.
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