Extraction of anthocyanins from freeze-dried puree (100 mg) for HPLC determination were carried out with 2 mL of acidified methanol (1% hydrochloridric acid) by placing samples in an ultrasonic bath (Bandelin Sonorex Digitech, Berlin, Germany) at room temperature for 15 min, then centrifuging for 15 min (Microspin,Grant-bio, England) (6596.2 ×g) and filtrated through 0.45 mm PTFE syringe filters. Extraction has been done in triplicate.