2.6.2. Oxidation fermentation test (Hugh and Leifson's test)
This experiment was done to confirm the ability of the isolates to ferment glucose with the production of acid and gas. This experiment was carried out in a medium with phenol red indicator. The medium was prepared and poured in to sterilized test tubes. After cooling the medium culture was inoculated into it and inoculated. After incubation the change in color was noted. The positive reaction was show by the color change from red to yellow.