demonstrated that the accumulation of NO and ROS is induced
before B. cinerea invasion. Importantly, although ROS accumulation
and disease area decreases markedly after treatment with an
NADPH oxidase inhibitor, the accumulation of ROS and the disease
area slightly increases after treatment with the NOS inhibitor LNAME,
suggesting that NO plays a role in basal defense against
B. cinerea and negatively regulates the B. cinerea-inducible oxidative
burst [43]. In the present study, we demonstrated the generation
of NO and subsequent accumulation of SNO proteins in
tomato leaves pretreated with EAWP, suggesting that regulation of
the redox flux by EAWP via NO signaling might contribute to the
inhibition of B. cinerea development in leaves.