The lues of Km, Vmax and kcat for CsnA were 7.29 mg mL−1, 7.48 μmol min−1, and 1.33 × 104 s−1, respectivvaely.
This recombinant chitosanase could hydrolyze chitosan efficiently into chitosan dimer, trimer, tetramer, pentamer and hexamer. This study demonstrates that functional screening based on metagenomics is a useful approach for isolating useful enzymes
containing