Method 1: For the immobilization of Lactobacillus in BC by adsorption-incubation method, G. xylinus bacteria were cultured in Hestrin-Schramm (HeS) medium composed of bacto-pepton e 0.5 w/v% (Oxoid, UK), glucose e 2 w/v%, yeast extract e 0.5 w/v%, citric acid e 0.115 w/v%, Na2HPO4 e 0.27 w/v%, MgSO4$7H2O e 0.05 w/v% and ethanol e 1 v/v% (Poch, Poland) added after sterilization of the base