The total yield of EPS produced by LAB mostly depends on the composition (carbon and nitrogen sources) of the growth
medium and the physical conditions at which the strains grow, i.e. temperature, pH of medium and incubation time (duration of fermentation). Not only the nature of the carbon source, sometimes the combination of sugars and their concentration units also have a stimulating effect on EPS biosynthesis. Some experimental studies showed that EPS production and growth were initially obtained higher with the supplementation of medium by skim milk and whey protein concentrate (WPC) or whey protein