2.1. Extraction
Extraction method was adopted from Sharifi and Hassani (2012)
using a reflux system. The barberries were first grounded by means
of a grinder (Black & Decker, USA) then were put into a solvent flask
including acidified ethanol and distilled water (1:3). The flask and
the condenser in theWater bath were exposed to a temperature of
50 C for 2 h. Then, the flask was separated from the system and
kept in the dark for 2 h. And finally, the obtained mixture was
filtered in a vacuum filter. The produced extract was condensed at
40 C to 15 brix by a rotary evaporator (IKA, Germany) and finally,
the condensed samples were stored in brown bottles.