Currently, the incidence of multidrug resistant organisms is increasing and compromising the treatment of a growing number of infectious diseases. As a result, there is an urgent need for developing new drugs which are effective against current antibiotic resistant pathogens. Actinomycetes have been proven as a potential source of bioactive compounds and richest source of secondary metabolites[41]. Isolation of antibacterial compounds from the freshwater environment is in advance interest in recent times to isolate novel bioactive actinomycetes. According to Sibanda et al., extracts from three freshwater actinomycetes showed inhibition to Gram negative bacterial strains compared to Gram positive bacterial strains[42]. These recent studies motivated to isolate antibiotic producing actinomycetes from fresh water and sediments of Lake Tana