2.2.2. Experimental design
The flocculation experiments on C. vulgaris culture broth were carried out in 250 ml beakers. The flocculant, flocculants mixture or flocculated biomass were added in varied concentrations. After the addition, the test sample was stirred at 200 rpm during 1 min and then left for sedimentation at room temperature. Each experiment had a control without flocculant. All experiments were performed in triplicate.