liquid had a pH 5.5. This inhibition increased with nisin
content of the films. Although the nisin released from films
suffered loss of activity, the gradual delivery of nisin still
contained in films controlled growth of L. innocua, helping
to reduce contamination along storage better than direct
addition.
It can be concluded that antimicrobial activity of the
films starch-based and containing nisin describe this hurdle
as adequate to act as a barrier to further product contamination.
Gradual release can also help to preclude microbial
proliferation better than nisin directly present in the
media. Additional studies must be performed to determine
if starch modification or additional changes in composition
(i.e. mixture of starch and lipids) might modify antimicrobial
activity and release producing changes in the possibilities
brought about by this hurdle