However, it is important to keep in mind that high NO levels
produced by iNOS are extensively released during inflamma-
tion and that, under these conditions, NF-jB is also activated.
Therefore, evaluation of ESW effect on NF-jB activation,
iNOS and other NF-jB-dependent gene expression is funda-
mental in assessing molecular mechanism(s) of the clinically
observed anti-inflammatory action of ESW.
In the present study, we examined the effect of ESW on the
modulation of nNOS catalytic activity, NO production, NF-
jB activation, and iNOS and TNF-a mRNA expression in
rat glioma C6 cells, a cell line taken as a cellular model because
it expresses both constitutive and inducible NOS.
However, it is important to keep in mind that high NO levelsproduced by iNOS are extensively released during inflamma-tion and that, under these conditions, NF-jB is also activated.Therefore, evaluation of ESW effect on NF-jB activation,iNOS and other NF-jB-dependent gene expression is funda-mental in assessing molecular mechanism(s) of the clinicallyobserved anti-inflammatory action of ESW.In the present study, we examined the effect of ESW on themodulation of nNOS catalytic activity, NO production, NF-jB activation, and iNOS and TNF-a mRNA expression inrat glioma C6 cells, a cell line taken as a cellular model becauseit expresses both constitutive and inducible NOS.
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