The present attempt aimed to optimize micropropagation protocols supplemented with different
concentrations and combinations of benzylaminopurine (BAP) (0, 2, 4, 6 mg L-1
) and indole acetic acid
(IAA) (0.5 and 1.0 mg L-1
). Exotic banana (Musa spp) genotypes GCTCV-215 (AAA), ‘Yangambi’
Yangambi Km-5 (AAA) and FHIA-23 (AAAA) were used in research work. Experiments were conducted at
Plant Tissue Culture Laboratory of Nuclear Institute of Agriculture (NIA), Tando Jam. Data collected for
in vitro shoot consists of the following parameters: days for bud initiation, rate of shoot proliferation
(%), number of multiple shoots, shoot length (cm) and fresh mass of shoot (g). Significant (p≤ 0.05)
variations were observed for varieties, treatments and varieties x treatment for all the parameters.
Synergistic effects of BAP and IAA were observed in GCTCV-215 and Yangambi Km-5. Out of various
treatments, best concentration for multiple shoot in short period of time for GCTCV-215 and Yangambi
Km-5 was found in 4.0 mg /l BAP + 0.5 mg L-1
IAA. Maximum fresh mass of shoot observed at same
concentration and combination of BAP and IAA and for shoot length combination of 4.0 mg L-1 BAP with
1.0 mg L-1
IAA was found to be most suitable for GCTCV-215 and Yangambi Km-5. FHIA-23, show better
performance in MS medium supplemented with only BAP at concentration 4.0 mg/L
-1
. After development
of root, in vitro plantlets were shifted from growth room to green house in polythene bags containing
garden soil and humus mixture in ratio (1:1).
Key words: Micropropagation efficiency, exotic musa genotype, growth regulators.
The present attempt aimed to optimize micropropagation protocols supplemented with differentconcentrations and combinations of benzylaminopurine (BAP) (0, 2, 4, 6 mg L-1) and indole acetic acid(IAA) (0.5 and 1.0 mg L-1). Exotic banana (Musa spp) genotypes GCTCV-215 (AAA), ‘Yangambi’Yangambi Km-5 (AAA) and FHIA-23 (AAAA) were used in research work. Experiments were conducted atPlant Tissue Culture Laboratory of Nuclear Institute of Agriculture (NIA), Tando Jam. Data collected forin vitro shoot consists of the following parameters: days for bud initiation, rate of shoot proliferation(%), number of multiple shoots, shoot length (cm) and fresh mass of shoot (g). Significant (p≤ 0.05)variations were observed for varieties, treatments and varieties x treatment for all the parameters.Synergistic effects of BAP and IAA were observed in GCTCV-215 and Yangambi Km-5. Out of varioustreatments, best concentration for multiple shoot in short period of time for GCTCV-215 and YangambiKm-5 was found in 4.0 mg /l BAP + 0.5 mg L-1IAA. Maximum fresh mass of shoot observed at sameconcentration and combination of BAP and IAA and for shoot length combination of 4.0 mg L-1 BAP with1.0 mg L-1IAA was found to be most suitable for GCTCV-215 and Yangambi Km-5. FHIA-23, show betterperformance in MS medium supplemented with only BAP at concentration 4.0 mg/L-1. After developmentof root, in vitro plantlets were shifted from growth room to green house in polythene bags containinggarden soil and humus mixture in ratio (1:1).Key words: Micropropagation efficiency, exotic musa genotype, growth regulators.
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