For these reasons tissue culture techniques are widely used to produce maximum mass from a single plant in a short span of time and also provide opportunities for germplasm conservation of important plants (Jagatheeswari and Ranganathan, 2012; Sivaram and Mukundan, 2003; Anbajhagan et al., 2010; Taware et al., 2010; Sabah and Rasha, 2013).