The research received ethical approval from Animal Care
Committee (ACC) of National Institute of Food Science
and Technology (NIFSAT). Later, National Institute of
Health (NIH) provided the researchers with 120 infectious
free Sprague dawley rats for the research purpose. The
rats were housed in the Animal Room of National Institute
of Food Science and Technology. Four different trials
depending on different types of diets i.e. trial-I (normal
diet), trial-II (high cholesterol diet), trial-III (high sucrose
diet) and trial-IV (high cholesterol + high sucrose diet)
were conducted. Therfore, 120 rats were divided in four
groups of thirty each. In each trial, rats were further divided
into three subgroups comprising of ten rats in each
subgroup. Rats were placed in standard polypropylene
cages with stainless steel top grill. Rats were acclimatized
by feeding basal diet (AIN-76A) for a period of one week.
At the initiation of study some rats were sacrificed to get
baseline values. The temperature (23 ± 2°C) and relative
humidity (55 ± 5%) were maintained throughout the experiment
with 12 hours light–dark period. Feed & drink
intake was recorded daily whilst body weight on weekly
basis throughout the experiment. Spilled diet and feces
were also collected.