2.2 Start-up and operation
A 10L-jacketed fermenter (Biostat B) equipped
with pH probe, stirrer, sampling ports and temperature
controller was used in this study. The working volume of
the bioreactor was maintained at 7 Litre and ran under
uncontrolled pH, which is without acid or base addition.
Experiments was carried out at thermophillic temperature
of 53o
C by circulating water from a thermostat through a
water jacket surrounding the bioreactor, and mixing was
aided by a mechanical stirrer set at a speed of 150rpm. The
system was started up as batch to achieve an active
acidifying culture by loading the substrate seeded with
POME, then sealed and purged with Nitrogen gas for 15
minutes. Semi-continuous feeding started from day 10,
where a known volume of slurry was withdrawn daily
from the reactor and replaced with fresh feedstock via the
slurry sampling ports equivalent to an HRT of 10 days. In
addition, approximately 50 ml of the sample was taken
daily from the bioreactor through the sampling port, which
2.2 Start-up and operation
A 10L-jacketed fermenter (Biostat B) equipped
with pH probe, stirrer, sampling ports and temperature
controller was used in this study. The working volume of
the bioreactor was maintained at 7 Litre and ran under
uncontrolled pH, which is without acid or base addition.
Experiments was carried out at thermophillic temperature
of 53o
C by circulating water from a thermostat through a
water jacket surrounding the bioreactor, and mixing was
aided by a mechanical stirrer set at a speed of 150rpm. The
system was started up as batch to achieve an active
acidifying culture by loading the substrate seeded with
POME, then sealed and purged with Nitrogen gas for 15
minutes. Semi-continuous feeding started from day 10,
where a known volume of slurry was withdrawn daily
from the reactor and replaced with fresh feedstock via the
slurry sampling ports equivalent to an HRT of 10 days. In
addition, approximately 50 ml of the sample was taken
daily from the bioreactor through the sampling port, which
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