A total of 0.1 mL of trichloroacetic acid-soluble extract sample was added 3.9 mL of distilled water, and as much as 5.5 mL Lowry reagent was added. The mixture was homogenized with a centrifuge (Type MX-305, Tomy Seiko, Co. Ltd., Tokyo, Japan) and incubated at room temperature for 10 minutes. Then 0.5 mL of Folin reagent was added and incubated at room temperature for 30 minutes, until the blue color was formed. Subsequently, the sample