.In this model system, b-carotene undergoes rapid discoloration
in the absence of an antioxidant, which results
in a reduction in absorbance of the test solution
with reaction time. This is because the coupled oxidation
of b-carotene and linoleic acid generates free radicals.
The linoleic acid free radical formed upon abstraction
of a hydrogen atom from one of its diallylic methylene
groups attacks highly unsaturated b-carotene molecules.
As b-carotene molecules lose their double bond, the system
loses its characteristic orange color, which can be
monitored spectrophotometrically (Wettasinghe and
Shahidi, 1999). The presence of an antioxidant hinders
the extent of bleaching by neutralizing the linoleate free
radical formed in the system. Progress of discoloration
process with time in differently treated test samples
was monitored spectrophotometerically, it was obse
.In this model system, b-carotene undergoes rapid discolorationin the absence of an antioxidant, which resultsin a reduction in absorbance of the test solutionwith reaction time. This is because the coupled oxidationof b-carotene and linoleic acid generates free radicals.The linoleic acid free radical formed upon abstractionof a hydrogen atom from one of its diallylic methylenegroups attacks highly unsaturated b-carotene molecules.As b-carotene molecules lose their double bond, the systemloses its characteristic orange color, which can bemonitored spectrophotometrically (Wettasinghe andShahidi, 1999). The presence of an antioxidant hindersthe extent of bleaching by neutralizing the linoleate freeradical formed in the system. Progress of discolorationprocess with time in differently treated test sampleswas monitored spectrophotometerically, it was obse
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