Eglantine rose (Rosa foetida) is an important wild, yellow rose species from Rosaceae family with tiny flowers. It is prop-agated by such methods as cutting and transplanting. Besides being time-consuming, these methods have usually someproblems. Therefore, in the current study the effect of different hormonal concentrations and culture medium on micro-propagation of this species was studied in tissue culture medium using axillary shoot explants. The experiment was carriedout as a factorial based on a randomized complete with three replications. The results of analysis of variance showed thatthe number and height of shoots had no significant difference in each explant in two culture media of MS and MS 1.2.However, different concentrations of BA and IBA had significant effect on the number and height of shoots. Means com-parison showed that the BA concentration of 4 mg/l with a mean shoot number of 3.75 and IBA concentration of 0.1 mg/lwith mean shoot number of 3.166 had the highest effect on shoot number. The BA concentrations of 4, 2 and 6 mg/l withshoot heights of 2.866, 2.833 and 2.375 cm, respectively and IBA concentrations of 0.1, 0.2 and 0 mg/l with shoot heightsof 3.06, 2.953 and 2.845 cm, respectively ranked in group A and had the highest shoot height. The BA concentration of 4mg/l and IBA concentration of 0.1 mg/l had the highest effect on both shoot number and height. The effect of environmenton rooting percentage and root number was highly significant. Also, the effect of IAA and IBA hormone on root numberwas significant. The best culture medium for increasing root number and percentage was found to be MS 1.2 with an IAAconcentration of 1.5 mg/l and IBA concentration of 1 mg/l.