However, it still requires validated tools able to inform state agencies, degradation and/or remediation of soil properties and functions, as well as monitoring networks. To date, soil characterization has generally been based on physical and chemical parameters. However, soil organisms are known to respond to multiple impacts and to react quickly to natural and anthropic stresses, and could, therefore, be used as early warning indicators. Moreover, soil biodiversity is recognized both by the Convention on Biological Diversity and by the European Commission in the thematic strategy for soil protection as essential for ecosystem functioning and the provisioning of soil services to humankind. Despite this recognition, biological tools are still rarely used in soil monitoring networks and their integration is quite recent.