COSMETICS
Materials and Methods
HA Diffusion Assay
Diffusion of tritiated HA with a molecu-
lar weight of about 50 kDa, 300 kDa, 800
kDa or 1500 kDa, respectively, through
dermatomed porcine ear skin was as-
sessed using Franz diffusion cells as pre-
viously described (11). In brief, full thick-
ness skin samples of visually intact skin
from the ear of 5-month old female do-
mestic pics were dermatomed to a thick-
ness of about 750 μm using an Acculan
GA 643 (Aesculap, Germany). The sam-
ples were mounted in modified Franz
static dermal penetration cells with the
external surface of the stratum corneum
facing the donor chamber. After 5 and
22h of HA application the level of radio-
activity in the receptor phase was deter-
mined and expressed as ng
∙
cm
-2
h
-1
which
served as a parameter for skin penetra-
tion