2. Materials and methods
2.1. Plant materials, culture system and nutrient media
2.1.1. Plant materials
Stem-node cuttings, 1.5 cm in length, were excised from 6 to
8cm in vitro potato plantlets derived from aseptic potato
meristems which had been cultured on 1/2 MS medium
(Murashige and Skoog, 1962) supplemented with 0.5mM
naphthaleneacetic acid (NAA), 0.3mM gibberellic acid (GA3),
3.7mM adenine sulfate, 10% coconut water, 0.5 g/l activated
charcoal, 20 g/l sucrose and 8 g/l agar for 30 days. Explants
were placed on solid or semi solid media depending on the
purpose of the experiments