Extraction and Fractionation of StreptomyceteMetabolites
fromSolid Cultures. In parallel, the selected Streptomyces isolate
was grown on SAA (Streptomyces Antibiotic Agar, [53])
since it was selected as optimum medium for high antifungal
activity expression. The cultures were incubated at 28∘C for 7
days. The medium around the formed streptomycete colony
in a radius of 2 cm was cut off and blended for three minutes.
The slurry was centrifuged at 4.000 g for 60 min and the
cell free supernatant was collected. For the determination
of antifungal activity, 400