i.e., by incubating 0.5 ml of culture supernatant with 0.5 ml
of 1% Avicel (microcrystalline cellulose) prepared in 0.05 M
sodium acetate buffer, pH 4.8 at 40 C for 1 h. After incubation,
released reducing sugars were measured by the DNS
method. One unit of Avicelase activity was defined as the
amount of enzyme that released 1 lmol of reducing sugars as
glucose equivalents min1 and the specific activity is the number
of units of enzyme activity per milligram of enzyme protein.