assessed by means of the hypo-osmotic swelling test
(HOST) as described by Buckett et al. [21]. Briefly, 5 ml of
semenwas diluted to 50 ml of a hypo-osmotic solution (100
mOsm) and incubated at 37C for 60 minutes. After incubation,
a smear was preparedm and 200 spermatozoa were
evaluated (1000 magnification) with brightfield microscopy.
Sperm with swollen or coiled tails were recorded