The lipase was optimally active at 45 °C and retained approximately 50% of its original
activity after 20 min of incubation at 55 °C. The enzyme was optimally active at pH 8.5. Mg2+, Cu2+, Ca2+, Hg2+, Al3+, and Fe3+ at
1 mM enhanced hydrolytic activity of the lipase. Interestingly, Hg2+ ions resulted in a maximal increase in lipase activity but Zn2+ and
Co2+ ions showed an antagonistic eVect on this enzyme.