3.5. Chemical and physical parameters
There was no statistical difference in temperature, dissolved oxygen, pH, total ammonia, nitrite and nitrate between the group fed a diet supplemented with Bacillus spp. probiotic (1.15 × 104 CFU·g− 1) and the control (p ≥ 0.05). The physical and chemical parameters found in this study are within the ideal conditions for the cultivation of L. vannamei ( Lourenço et al., 2009).