.Introduction
The increasing amount of interest being given to attaining wellness through nutrition as well as the results of scientific research, which has demonstrated the potential health benefits of particular biologically active components (nutraceutical compounds), are the main factors supporting the demand for foods with evident effects on human health. These have been generally defined as “functional foods”. There has been a particular focus of attention on fresh red fruits and their derivatives (juice, nectars, mousses and purees), due to their possible health benefits as dietary antioxidant, anti-mutagenic, and chemo-preventive nutraceuticals that can possibly contribute to reducing chronic diseases. The functional properties of red fruit-based foodstuffs are mainly related to the concentration or presence of anthocyanins, polyphenols, tannins and vitamins, as well as bioactive molecules, which are capable of ensuring antioxidant properties, colour intensity, astringency and nutritional value. Unfavourably, bioactive compounds are quickly affected by exogenic factors such as oxygen, light, and, in particular, both pH as well as temperature. Despite of the optimization of the process conditions and the mild technological solution applied in the case of red-fruit derivatives, the thermal stabilization of these products induces the degradation of the bioactive compounds with an evident effect on both the quality perception (colour, taste, aroma) as well as the nutritional value. The natural pigments, mainly represented by the anthocyanidins, usually red or blue or variations, due to their high reactivity, readily degrade and form colourless or undesirable brown-coloured compounds. Many factors affect the stability of the anthocyanins, including temperature, pH, oxygen, enzymes, the presence of co-pigments and metallic ions, ascorbic acid, sulphur dioxide as well as sugars and their degradation products. During pasteurization and storage, several red fruit derivatives lose their bright red colour and become a dirty- brown one. Similarly, the behaviour of the polyphenol content is similar to that observed for the anthocyanin content in several liquid, semi-solid or solid foodstuffs. The challenge to preserve the polyphenols and anthocyanins suggests the application of the high hydrostatic pressure for the sanitation of the products rich in bioactive compounds, such as red-fruits derivatives. In current literature, the scientific results reporting the stability or preservation of these compounds are contradictory. Several authors have reported that the anthocyanins of different liquid foods (red-fruit juices) are stable to HHP treatment at moderate temperatures. For example, in pomegranate juice at room temperature, the nutraceutical and sensorial properties are strictly related to the anthocyanin and polyphenol content. The concentration of these molecules decreases with the intensity of the treatment in terms of pressure level and processing time [1]. Therefore, both the higher pressure levels as well as longer processing times cause a decrease of the anthocyanin content. In contrast, some authors have reported increased extractability of coloured pigments from the pulp suspended in the clear juices at extreme pressures. However, the observed stability of these molecules could be transient, due to the pressure and temperature levels applied during the experiments, which cannot induce an irreversible inactivation of the enzymes involved in the degradation of the natural pigments [1, 2, 3]. The aim of this paper is to investigate the stability of bioactive compounds and, in particular, of the total anthocyanin and polyphenol content of high pressure treated red fruit-based products. Liquid (pomegranate juice) and semi-solid foods (strawberry, wild strawberry mousses) were processed in high hydrostatic pressure cycles, which were optimized in previous experiments. The products, microbiologically stable, were stored under refrigerated conditions for 3 months, with the bioactive molecules being determined weekly.
.Introduction The increasing amount of interest being given to attaining wellness through nutrition as well as the results of scientific research, which has demonstrated the potential health benefits of particular biologically active components (nutraceutical compounds), are the main factors supporting the demand for foods with evident effects on human health. These have been generally defined as “functional foods”. There has been a particular focus of attention on fresh red fruits and their derivatives (juice, nectars, mousses and purees), due to their possible health benefits as dietary antioxidant, anti-mutagenic, and chemo-preventive nutraceuticals that can possibly contribute to reducing chronic diseases. The functional properties of red fruit-based foodstuffs are mainly related to the concentration or presence of anthocyanins, polyphenols, tannins and vitamins, as well as bioactive molecules, which are capable of ensuring antioxidant properties, colour intensity, astringency and nutritional value. Unfavourably, bioactive compounds are quickly affected by exogenic factors such as oxygen, light, and, in particular, both pH as well as temperature. Despite of the optimization of the process conditions and the mild technological solution applied in the case of red-fruit derivatives, the thermal stabilization of these products induces the degradation of the bioactive compounds with an evident effect on both the quality perception (colour, taste, aroma) as well as the nutritional value. The natural pigments, mainly represented by the anthocyanidins, usually red or blue or variations, due to their high reactivity, readily degrade and form colourless or undesirable brown-coloured compounds. Many factors affect the stability of the anthocyanins, including temperature, pH, oxygen, enzymes, the presence of co-pigments and metallic ions, ascorbic acid, sulphur dioxide as well as sugars and their degradation products. During pasteurization and storage, several red fruit derivatives lose their bright red colour and become a dirty- brown one. Similarly, the behaviour of the polyphenol content is similar to that observed for the anthocyanin content in several liquid, semi-solid or solid foodstuffs. The challenge to preserve the polyphenols and anthocyanins suggests the application of the high hydrostatic pressure for the sanitation of the products rich in bioactive compounds, such as red-fruits derivatives. In current literature, the scientific results reporting the stability or preservation of these compounds are contradictory. Several authors have reported that the anthocyanins of different liquid foods (red-fruit juices) are stable to HHP treatment at moderate temperatures. For example, in pomegranate juice at room temperature, the nutraceutical and sensorial properties are strictly related to the anthocyanin and polyphenol content. The concentration of these molecules decreases with the intensity of the treatment in terms of pressure level and processing time [1]. Therefore, both the higher pressure levels as well as longer processing times cause a decrease of the anthocyanin content. In contrast, some authors have reported increased extractability of coloured pigments from the pulp suspended in the clear juices at extreme pressures. However, the observed stability of these molecules could be transient, due to the pressure and temperature levels applied during the experiments, which cannot induce an irreversible inactivation of the enzymes involved in the degradation of the natural pigments [1, 2, 3]. The aim of this paper is to investigate the stability of bioactive compounds and, in particular, of the total anthocyanin and polyphenol content of high pressure treated red fruit-based products. Liquid (pomegranate juice) and semi-solid foods (strawberry, wild strawberry mousses) were processed in high hydrostatic pressure cycles, which were optimized in previous experiments. The products, microbiologically stable, were stored under refrigerated conditions for 3 months, with the bioactive molecules being determined weekly.
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.Introduction
The increasing amount of interest being given to attaining wellness through nutrition as well as the results of scientific research, which has demonstrated the potential health benefits of particular biologically active components (nutraceutical compounds), are the main factors supporting the demand for foods with evident effects on human health. These have been generally defined as “functional foods”. There has been a particular focus of attention on fresh red fruits and their derivatives (juice, nectars, mousses and purees), due to their possible health benefits as dietary antioxidant, anti-mutagenic, and chemo-preventive nutraceuticals that can possibly contribute to reducing chronic diseases. The functional properties of red fruit-based foodstuffs are mainly related to the concentration or presence of anthocyanins, polyphenols, tannins and vitamins, as well as bioactive molecules, which are capable of ensuring antioxidant properties, colour intensity, astringency and nutritional value. Unfavourably, bioactive compounds are quickly affected by exogenic factors such as oxygen, light, and, in particular, both pH as well as temperature. Despite of the optimization of the process conditions and the mild technological solution applied in the case of red-fruit derivatives, the thermal stabilization of these products induces the degradation of the bioactive compounds with an evident effect on both the quality perception (colour, taste, aroma) as well as the nutritional value. The natural pigments, mainly represented by the anthocyanidins, usually red or blue or variations, due to their high reactivity, readily degrade and form colourless or undesirable brown-coloured compounds. Many factors affect the stability of the anthocyanins, including temperature, pH, oxygen, enzymes, the presence of co-pigments and metallic ions, ascorbic acid, sulphur dioxide as well as sugars and their degradation products. During pasteurization and storage, several red fruit derivatives lose their bright red colour and become a dirty- brown one. Similarly, the behaviour of the polyphenol content is similar to that observed for the anthocyanin content in several liquid, semi-solid or solid foodstuffs. The challenge to preserve the polyphenols and anthocyanins suggests the application of the high hydrostatic pressure for the sanitation of the products rich in bioactive compounds, such as red-fruits derivatives. In current literature, the scientific results reporting the stability or preservation of these compounds are contradictory. Several authors have reported that the anthocyanins of different liquid foods (red-fruit juices) are stable to HHP treatment at moderate temperatures. For example, in pomegranate juice at room temperature, the nutraceutical and sensorial properties are strictly related to the anthocyanin and polyphenol content. The concentration of these molecules decreases with the intensity of the treatment in terms of pressure level and processing time [1]. Therefore, both the higher pressure levels as well as longer processing times cause a decrease of the anthocyanin content. In contrast, some authors have reported increased extractability of coloured pigments from the pulp suspended in the clear juices at extreme pressures. However, the observed stability of these molecules could be transient, due to the pressure and temperature levels applied during the experiments, which cannot induce an irreversible inactivation of the enzymes involved in the degradation of the natural pigments [1, 2, 3]. The aim of this paper is to investigate the stability of bioactive compounds and, in particular, of the total anthocyanin and polyphenol content of high pressure treated red fruit-based products. Liquid (pomegranate juice) and semi-solid foods (strawberry, wild strawberry mousses) were processed in high hydrostatic pressure cycles, which were optimized in previous experiments. The products, microbiologically stable, were stored under refrigerated conditions for 3 months, with the bioactive molecules being determined weekly.
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