3. Results
When the pollen of bottle gourd cultivar ‘Kachidoki 2′ was used to pollinate watermelon, the fruit set rate was 57.1% (Table 1) as compared with a fruit set rate of 65.0% by pollinating with watermelon pollen. Although the fruit set rate using bottle gourd pollen was slightly lower than the rate using watermelon pollen, this relatively high rate was higher than expected. The pistillate flowers of watermelon pollinated by bottle gourd pollen developed fruit. In contrast, when the pollen of bitter melon and loofa were used to pollinate watermelon, none of the watermelon plants set fruit.
Table 1.
Watermelon fruit set rate after pollination by different species from Cucurbitaceae family.
Pollen source Number of pollination Number of fruit set Fruit set rate (%)
Bottle gourd (Kachidoki 2)a
28b
16 57.1
Bitter melon (Goya) 18c
0 0.0
Loofa (Onaga hechima) 23c
0 0.0
Watermelon (Fujihikari TR) 20c
13 65.0
a
(): Cultivar name.
b
Pollination of watermelon one day pre-anthesis.
c
Pollination of watermelon on the day of anthesis.
Table options
We investigated the storage conditions for bottle gourd pollen by comparing the fruit set ability using the following two methods: (1) preservation of staminate flowers and (2) preservation of pollen. When staminate flowers of bottle gourd were harvested and stored at 10 °C, the fruit set rate from the stored flowers was 22.2% (Table 2). When staminate flowers were stored at 4 °C for 24 h or 72 h, the fruit set rate was 53.9% and 40.0%, respectively. Fruit did not set when the staminate flowers of bottle gourd were stored at 15 °C. In contrast, bottle gourd pollen stored at −30 °C had no fruit setting ability even if stored for only a day. Thus, preservation of pollen collected from staminate bottle gourd flowers was difficult, and a difference in fruit setting ability was observed depending on whether staminate flowers or stored pollen.
Table 2.
Relationship between watermelon fruit set rate and bottle gourd pollen preservation method.
Preserved materials (bottle gourd) Preservation conditions
________________________________________
Temperature (°C) Period (h) Number of pollinations Fruit set rate (%)
Staminate flower with pollen 15 24 13 0.0
Staminate flower with pollen 10 24 9 22.2
Staminate flower with pollen 4 24 13 53.9
Staminate flower with pollen 4 72 5 40.0
Pollen −30 24 5 0.0
Pollen −30 >7 daysa
9 0.0
a
Preservation for more than seven days.
3. Results
When the pollen of bottle gourd cultivar ‘Kachidoki 2′ was used to pollinate watermelon, the fruit set rate was 57.1% (Table 1) as compared with a fruit set rate of 65.0% by pollinating with watermelon pollen. Although the fruit set rate using bottle gourd pollen was slightly lower than the rate using watermelon pollen, this relatively high rate was higher than expected. The pistillate flowers of watermelon pollinated by bottle gourd pollen developed fruit. In contrast, when the pollen of bitter melon and loofa were used to pollinate watermelon, none of the watermelon plants set fruit.
Table 1.
Watermelon fruit set rate after pollination by different species from Cucurbitaceae family.
Pollen source Number of pollination Number of fruit set Fruit set rate (%)
Bottle gourd (Kachidoki 2)a
28b
16 57.1
Bitter melon (Goya) 18c
0 0.0
Loofa (Onaga hechima) 23c
0 0.0
Watermelon (Fujihikari TR) 20c
13 65.0
a
(): Cultivar name.
b
Pollination of watermelon one day pre-anthesis.
c
Pollination of watermelon on the day of anthesis.
Table options
We investigated the storage conditions for bottle gourd pollen by comparing the fruit set ability using the following two methods: (1) preservation of staminate flowers and (2) preservation of pollen. When staminate flowers of bottle gourd were harvested and stored at 10 °C, the fruit set rate from the stored flowers was 22.2% (Table 2). When staminate flowers were stored at 4 °C for 24 h or 72 h, the fruit set rate was 53.9% and 40.0%, respectively. Fruit did not set when the staminate flowers of bottle gourd were stored at 15 °C. In contrast, bottle gourd pollen stored at −30 °C had no fruit setting ability even if stored for only a day. Thus, preservation of pollen collected from staminate bottle gourd flowers was difficult, and a difference in fruit setting ability was observed depending on whether staminate flowers or stored pollen.
Table 2.
Relationship between watermelon fruit set rate and bottle gourd pollen preservation method.
Preserved materials (bottle gourd) Preservation conditions
________________________________________
Temperature (°C) Period (h) Number of pollinations Fruit set rate (%)
Staminate flower with pollen 15 24 13 0.0
Staminate flower with pollen 10 24 9 22.2
Staminate flower with pollen 4 24 13 53.9
Staminate flower with pollen 4 72 5 40.0
Pollen −30 24 5 0.0
Pollen −30 >7 daysa
9 0.0
a
Preservation for more than seven days.
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