2.4.6. Effects of aw on long-term conidial survival at 40 and 50 _C under anaerobic conditions
Samples of conidia (0.6 g) were initially dried over drierite in 125-mL glass jars for 3 days at 25 _C and then transferred to foil pouches. Conidial aw was then adjusted to different levels via the following treatments: (1) addition of sachet 504E (O2 scavenger/ CO2 and H2O generator), (2) addition of sachet RP-3 K (O2 scavenger/H2O generator), (3) addition of sachet RP-3A (O2/H2O scavenger), and (4) hydration of conidia for 6.5 h over 30 mL distilled water (in glass jar) prior to addition of sachet RP-3A. For each treatment, three independently prepared replicate pouches were destructively evaluated after 7 days at 25 _C (equilibration period) in order to determine viabilities of conidia prior to high-temperature storage, and four pouches were destructively evaluated in assessments following long-term storage for 3 months at 50 _C or 16 months at 40 _C. The experiment was repeated on a different date, the only difference being that drierite (10 g) was also enclosed in each pouch with a 504E sachet (treatment 1).
2.4.6. Effects of aw on long-term conidial survival at 40 and 50 _C under anaerobic conditionsSamples of conidia (0.6 g) were initially dried over drierite in 125-mL glass jars for 3 days at 25 _C and then transferred to foil pouches. Conidial aw was then adjusted to different levels via the following treatments: (1) addition of sachet 504E (O2 scavenger/ CO2 and H2O generator), (2) addition of sachet RP-3 K (O2 scavenger/H2O generator), (3) addition of sachet RP-3A (O2/H2O scavenger), and (4) hydration of conidia for 6.5 h over 30 mL distilled water (in glass jar) prior to addition of sachet RP-3A. For each treatment, three independently prepared replicate pouches were destructively evaluated after 7 days at 25 _C (equilibration period) in order to determine viabilities of conidia prior to high-temperature storage, and four pouches were destructively evaluated in assessments following long-term storage for 3 months at 50 _C or 16 months at 40 _C. The experiment was repeated on a different date, the only difference being that drierite (10 g) was also enclosed in each pouch with a 504E sachet (treatment 1).
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