The medium polar–apolar column combination generated a well-known elution order: in the first dimension, considering FAMEs with an equivalent C-number, retention times increased with the number of double bonds, while the opposite elution order occurred in the second dimension. Specifically, saturated FAMEs were located in the higher parts of the chromatogram followed by mono-unsaturates, di-unsaturates, etc. All the saturated FAMEs (C8:0 –C24:0 ) have been reported in Fig. 5, while the earliest and latest-eluting FAME have been reported for the other classes of compounds, depending on the double-bond number (from one to six). Apart from the chromatogram positions, identification was performed through MS-database searching. It must be noted that the chromatogram in Fig. 5 has been wrap-around corrected using a specific software function. Under the proposed operational con- ditions wrap-around was inevitable, since the dead time is approx. 5.6 s; however, it had no overall negative effect on the structure of the chromatogram. In general, extensive wrap-around can repre- sent a problem especially for samples of higher complexity.