At least 15 ml of oral fluid sample was collected from each pen at each time point (pre- and post-challenge). Powder-free nitrile gloves were worn during the collection process and changed between each pen. Samples were stored at 20 8C until assayed.
At least 15 ml of oralfluid sample was collected from each pen at each time point(pre- and post-challenge). Powder-free nitrile gloves wereworn during the collection process and changed betweeneach pen. Samples were stored at 20 8C until assayed.