Inoculation with low-LAB + CNC culture also increased (p < 0.001) the fat acidity of salami, both before and after drying. Other studies on the salami reported a similar increase in fat acidity as a result of adding LAB + CNC cultures. Bacterial lipolysis has been related with staphylococci activity and, to a lesser extent, with micrococci and some strains of L. sakei and L. curvatus, although endogenous lipolysis also contributes to the formation of free fatty acids in a salami. Free fatty acids, especially unsaturated, degrade to form aldehydes, ketones and other secondary compounds that strongly contribute to aroma and flavour in fermented meat. The starter cultures used were selected CNC strains of S. carnosus and K. varians with protease and lipolytic activities, and therefore capable of increasing the level of free fatty acids and non-protein nitrogen compounds in a salami. This was confirmed by the strong correlation (p < 0.001) found between Micrococcaceae counts, the proteolytic index (R = 0.80) and fat acidity (R = 0.78), while the LAB counts were not correlated (p > 0.05) with either of these ripen- ing properties.
Inoculation with low-LAB + CNC culture also increased (p < 0.001) the fat acidity of salami, both before and after drying. Other studies on the salami reported a similar increase in fat acidity as a result of adding LAB + CNC cultures. Bacterial lipolysis has been related with staphylococci activity and, to a lesser extent, with micrococci and some strains of L. sakei and L. curvatus, although endogenous lipolysis also contributes to the formation of free fatty acids in a salami. Free fatty acids, especially unsaturated, degrade to form aldehydes, ketones and other secondary compounds that strongly contribute to aroma and flavour in fermented meat. The starter cultures used were selected CNC strains of S. carnosus and K. varians with protease and lipolytic activities, and therefore capable of increasing the level of free fatty acids and non-protein nitrogen compounds in a salami. This was confirmed by the strong correlation (p < 0.001) found between Micrococcaceae counts, the proteolytic index (R = 0.80) and fat acidity (R = 0.78), while the LAB counts were not correlated (p > 0.05) with either of these ripen- ing properties.
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Inoculation with low-LAB + CNC culture also increased (p < 0.001) the fat acidity of salami, both before and after drying. Other studies on the salami reported a similar increase in fat acidity as a result of adding LAB + CNC cultures. Bacterial lipolysis has been related with staphylococci activity and, to a lesser extent, with micrococci and some strains of L. sakei and L. curvatus, although endogenous lipolysis also contributes to the formation of free fatty acids in a salami. Free fatty acids, especially unsaturated, degrade to form aldehydes, ketones and other secondary compounds that strongly contribute to aroma and flavour in fermented meat. The starter cultures used were selected CNC strains of S. carnosus and K. varians with protease and lipolytic activities, and therefore capable of increasing the level of free fatty acids and non-protein nitrogen compounds in a salami. This was confirmed by the strong correlation (p < 0.001) found between Micrococcaceae counts, the proteolytic index (R = 0.80) and fat acidity (R = 0.78), while the LAB counts were not correlated (p > 0.05) with either of these ripen- ing properties.
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