A serial dilution (5e30 mg/ml) of standard gallic acid purchased
from Fluka (USA) was prepared in H2O to generate a calibration
curve (r > 0.999). The sample was mixed with water, Folin-
Ciocalteu reagent (Carlo erba, Italy) and 2% Na2CO3 (Fisher, UK)
prior to incubation for 1 h at ambient conditions. Absorbencies
were recorded at 750 nm using a microplate reader. The TPC was
calculated and expressed as g of gallic acid equivalents per 100 g of
extract (g GAE/100 g extract). All measurements were performed in
triplicate