in three treatments: treatment LR: live cooked crabs (n ¼ 15);
treatment DR: overnight dead cooked crabs (n ¼ 15); and treatment
LF: live cooked crabs frozen at 20 C during 3 months (n ¼ 15). All
specimens were stored at 3.4 0.9 C during 13 days, and peri-
odically three crabs per treatment were randomly sampled. Muscle
(claws and legs) and brown meat (hepatopancreas and gonads)
were separated and subjected to sensorial, physical and microbio-
logical analyses. Each tissue was homogenised with a grinder,
vacuum-packed and stored at 80 C until further chemical anal-
yses. All analyses were carried out in duplicate.