Three subspecies of B. canis have been proposed (Uilenberg et al., 1989) and detected in many countries on the world (Martinod et al., 1986; Uilenberg et al., 1989;Matjila et al., 2004). However the differentiation between these subspecies is impossible by direct examination of blood smears. In the present study we developed a Real Time PCR for specific detection of B. canis canis, B. canis