2.7. Resistant starch (RS)
Resistant starch content was determined according to Gõni,
Garcia-Diz, Manas, and Saura-Calixto (1996). This method involved
removal of protein with pepsin (40 C, 1 h, pH 1.5), incubation with
a-amylase (37 C, 16 h) to hydrolyse digestible starch, treatment of
the residues with 2 M KOH to solubilise RS and finally incubation
with amyloglucosidase (60 C, 45 min, pH 4.75) to hydrolyse RS.
Free glucose was determined using the glucose oxidase assay
amylose content was calculated based on the standard curve.