Alyxia reinwardtii Blume is used in traditional medicine for a long time. This
study was carried out to investigate the standardization parameters by qualitative and
quantitative analyses as well as coumarin content of A. reinwardtii inner bark. Loss on
drying, total ash, acid-insoluble ash, water soluble extractives, ethanol soluble extractives
and moisture contents were found to be 8.1 ± 0.2, 7.6 ± 0.1, 1.4 ± 0.1, 16.5 ± 0.1 , 8.9 ±
0.3 and 11.9 ± 0.2% by dry weight, respectively. Coumarin in dichloromethane extract of
A. reinwardtii inner bark was analysed by thin layer chromatography (TLC) using silica gel
60 F254 as stationary phase, hexane and ethyl acetate (1 : 1) as mobile phase. The content
was evaluated by image analysis using image J software. The regression line of method was
polynomial in range of 0.075 - 1.2 mg/spot, and correlation coefficients (R2) were 0.99.
The repeatability and intermediate precisions were between 0.5 - 7.1% RSD. The percent
recovery was found to be 96.8 - 107.3%. The robustness evaluated by slightly variation in
mobile phase ratio was 1.3% RSD. Limit of detection (LOD) and limit of quantitation
(LOQ) were 0.05 and 0.16 mg/spot respectively. The coumarin content in A. reinwardtii
inner bark crude drug was found to be 0.75 ± 0.01% by dry weight. This study provided
pharmacognostic specification toward fundamental standardization of A. reinwardtii inner
bark. Additionally, the simple TLC with image analysis can be applied to quantitatively
determine coumarin in plant material.
Alyxia reinwardtii Blume is used in traditional medicine for a long time. Thisstudy was carried out to investigate the standardization parameters by qualitative andquantitative analyses as well as coumarin content of A. reinwardtii inner bark. Loss ondrying, total ash, acid-insoluble ash, water soluble extractives, ethanol soluble extractivesand moisture contents were found to be 8.1 ± 0.2, 7.6 ± 0.1, 1.4 ± 0.1, 16.5 ± 0.1 , 8.9 ±0.3 and 11.9 ± 0.2% by dry weight, respectively. Coumarin in dichloromethane extract ofA. reinwardtii inner bark was analysed by thin layer chromatography (TLC) using silica gel60 F254 as stationary phase, hexane and ethyl acetate (1 : 1) as mobile phase. The contentwas evaluated by image analysis using image J software. The regression line of method waspolynomial in range of 0.075 - 1.2 mg/spot, and correlation coefficients (R2) were 0.99.The repeatability and intermediate precisions were between 0.5 - 7.1% RSD. The percentrecovery was found to be 96.8 - 107.3%. The robustness evaluated by slightly variation inmobile phase ratio was 1.3% RSD. Limit of detection (LOD) and limit of quantitation(LOQ) were 0.05 and 0.16 mg/spot respectively. The coumarin content in A. reinwardtiiinner bark crude drug was found to be 0.75 ± 0.01% by dry weight. This study providedpharmacognostic specification toward fundamental standardization of A. reinwardtii innerbark. Additionally, the simple TLC with image analysis can be applied to quantitativelydetermine coumarin in plant material.
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