Aliquots of 20 g of sauerkraut were mixed with 20 ml distilled
water and homogenized using an Ultra-Turrax T25 basic (Ika
Werke). Total genomic DNAwas extracted using a fast-DNA spin kit
for soil (MP Biomedical) according to the manufacturer’s instructions.
Microbial genomic DNA isolated from sauerkraut was
directly used to amplify the V6-V8 region of the bacterial 16S rRNA
gene, using primers described by Nübel et al. (1996). PCR-DGGE
was performed according to Lima et al. (2012).