To test the specificity of EHP-primers, PCR assays were performed
with DNA templates from 2 parasitic diseases: (1) cotton
shrimp disease, P. monodon collected from Madagascar in 2006,
these shrimp showed a whitish muscle, their DNA were positive
for a Pleistophora-like microsporidium determined by small subunit
rRNA gene sequencing (Genbank No. KP825331); (2) ameoba
disease, these P. vannamei suffered substantial mortality and were
found, through histological examination, to be infected with
amoeba (species not determined).
To test the specificity of EHP-primers, PCR assays were performedwith DNA templates from 2 parasitic diseases: (1) cottonshrimp disease, P. monodon collected from Madagascar in 2006,these shrimp showed a whitish muscle, their DNA were positivefor a Pleistophora-like microsporidium determined by small subunitrRNA gene sequencing (Genbank No. KP825331); (2) ameobadisease, these P. vannamei suffered substantial mortality and werefound, through histological examination, to be infected withamoeba (species not determined).
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