Cultures were inoculated at 25±2oC under cool fluorescent light (1500-2000 Lux) with a 16 hr/ 8hr light- dark cycle. Each treatment consisted of minimum 15 explants and all experiments were conducted under sterile conditions. The inoculation tools like forceps, needles, scissors, blades, scalpels and others were thoroughly autoclaved. Culture areas, glasswares and all other tools used were properly sterilized with UV Light and floor surfaces were swabble with 95% ethyl alcohol, inoculation tools were flamed from time to time.